胸腺素β4(TMSB4X)活性蛋白
Active Thymosin Beta 4 (TMSB4X)
Tb4; FX; PTMB4; TB4X; TMSB4; Thymosin,Beta 4,X-Linked; TB-500; Seraspenide; Hematopoietic system regulatory peptide
- 編號UAPB609Hu03
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點6.0
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Thymosin beta 4 (T beta 4; also TB4X and Fx) is a 5.0 kDa member of the beta-thymosin family of molecules. It Plays an important role in the organization of the cytoskeleton. It is reported that TMSB4X can combinant with PFN1 to inhibit actin polymerization. A binding ELISA assay was conducted to detect the interaction of recombinant human TMSB4X and recombinant human PFN1. Briefly, TMSB4X were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to PFN1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TMSB4X pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TMSB4X and PFN1 was shown in Figure 1, the EC50?for this effect is 2.98 ng/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB609Hu01 | 胸腺素β4(TMSB4X)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB609Hu61 | 胸腺素β4(TMSB4X)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPB609Hu03 | 胸腺素β4(TMSB4X)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB609Hu01 | 胸腺素β4(TMSB4X)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPB609Hu03 | 胸腺素β4(TMSB4X)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPB609Hu61 | 胸腺素β4(TMSB4X)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB609Hu02 | 胸腺素β4(TMSB4X)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UCPB609Hu11 | 胸腺素β4(TMSB4X)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPB609Hu21 | 胸腺素β4(TMSB4X)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAB609Hu03 | 胸腺素β4(TMSB4X)多克隆抗體 | WB |
| UPAB609Hu01 | 胸腺素β4(TMSB4X)多克隆抗體 | Testing in progress. |
| UPAB609Hu08 | 胸腺素β4(TMSB4X)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAB609Hu71 | 胸腺素β4(TMSB4X)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| UMAB609Hu21 | 胸腺素β4(TMSB4X)單克隆抗體 | WB; IHC; ICC; IP. |
| UCEB609Hu | 胸腺素β4(TMSB4X)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB609Hu | 胸腺素β4(TMSB4X)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Scientific Reports | Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD) [33469081] |
