VGF神經(jīng)生長因子誘導蛋白(VGF)活性蛋白
Active VGF Nerve Growth Factor Inducible (VGF)
NERP1; NERP2; Neuro-Endocrine Specific Protein VGF; Neuroendocrine Regulatory Peptide
- 編號UAPB166Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 80%
- 等電點4.0
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Neurosecretory protein VGF is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The protein shares similarities with the secretogranin/chromogranin family, however, its exact function is not known. Besides, Early Growth Response Protein 1 (EGR1) has been identified as an interactor of VGF, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse VGF and recombinant mouse EGR1. Briefly, EGR1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to VGF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti- EGR1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450/630nm immediately. The binding activity of VGF and EGR1 was shown in Figure 1, the ED50?for this effect is 0.473 ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPB166Mu61 | VGF神經(jīng)生長因子誘導蛋白(VGF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB166Mu02 | VGF神經(jīng)生長因子誘導蛋白(VGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB166Mu01 | VGF神經(jīng)生長因子誘導蛋白(VGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPB166Mu61 | VGF神經(jīng)生長因子誘導蛋白(VGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPB166Mu01 | VGF神經(jīng)生長因子誘導蛋白(VGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB166Mu01 | VGF神經(jīng)生長因子誘導蛋白(VGF)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAB166Mu02 | VGF神經(jīng)生長因子誘導蛋白(VGF)多克隆抗體 | WB; IHC; ICC; IP. |
| USEB166Mu | VGF神經(jīng)生長因子誘導蛋白(VGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB166Mu | VGF神經(jīng)生長因子誘導蛋白(VGF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Neuroendocrinology | Proteomic analysis of cerebrospinal fluid from obese women with idiopathic intracranial hypertension: a new approach for identifying new candidates in the pathogenesis of obesity [PubMed: 22296024] |
| J Psychiatr Res | Low VGF is associated with executive dysfunction in patients with major depressive disorder [Pubmed:35738161] |
