血管內(nèi)皮生長因子C(VEGFC)活性蛋白
Active Vascular Endothelial Growth Factor C (VEGFC)
VEGF-C; Flt4-L; VRPL; Vascular Endothelial Growth Factor-Related Protein
- 編號(hào)UAPA145Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)8.2
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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Figure. Western Blot; Sample: Recombinant VEGFC, Human.
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Vascular endothelial growth factor C (VEGFC) is a protein that is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family. It plays key roles in the physiology and pathology of many aspects of the cardiovascular system, including vasculogenesis, hematopoiesis, angiogenesis and vascular permeability. To test the effect of VEGFC on cell proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 2,000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM?prior to the addition of various concentrations of VEGFC. After incubated for 72h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C. The dose-effect curve of VEGFC was shown in Figure 1. It was obvious that VEGFC significantly promoted cell proliferation of ECV304 cells. The ED50 for this effect is typically 0.304 to 1.339μg/mL.
用法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA145Hu61 | 血管內(nèi)皮生長因子C(VEGFC)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA145Hu01 | 血管內(nèi)皮生長因子C(VEGFC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA145Hu02 | 血管內(nèi)皮生長因子C(VEGFC)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA145Hu62 | 血管內(nèi)皮生長因子C(VEGFC)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA145Hu63 | 血管內(nèi)皮生長因子C(VEGFC)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA145Hu61 | 血管內(nèi)皮生長因子C(VEGFC)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA145Hu01 | 血管內(nèi)皮生長因子C(VEGFC)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA145Hu01 | 血管內(nèi)皮生長因子C(VEGFC)多克隆抗體 | WB. |
| UMAA145Hu23 | 血管內(nèi)皮生長因子C(VEGFC)單克隆抗體 | IHC |
| UMAA145Hu24 | 血管內(nèi)皮生長因子C(VEGFC)單克隆抗體 | IHC |
| UMAA145Hu22 | 血管內(nèi)皮生長因子C(VEGFC)單克隆抗體 | IHC |
| UMAA145Hu21 | 血管內(nèi)皮生長因子C(VEGFC)單克隆抗體 | IHC |
| UMEA145Hu | 血管內(nèi)皮生長因子C(VEGFC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA145Hu | 血管內(nèi)皮生長因子C(VEGFC)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA145Hu | 血管內(nèi)皮生長因子C(VEGFC)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA145Hu | 血管內(nèi)皮生長因子C(VEGFC)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA145Hu01 | 血管內(nèi)皮生長因子C(VEGFC)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Cellular Biochemistry | Alternatively activated RAW264. 7 macrophages enhance tumor lymphangiogenesis in mouse lung adenocarcinoma [PubMed: 19241443] |
| genetics and molecular research | Expression of COX-2 and VEGF-C in cholangiocarcinomas at different clinical and pathological stages [PubMed: 26125824] |
| Experimental and Therapeutic | Fenofibrate inhibits the expression of VEGFC and VEGFR-3 in retinal pigmental epithelial cells exposed to hypoxia [PubMed: 26622498] |
| Am J Pathol | IL-10 Indirectly Regulates Corneal Lymphangiogenesis and Resolution of Inflammation via Macrophages [PubMed: 26608451] |
| PLoS One. | Interleukin-6 Induces Vascular Endothelial Growth Factor-C Expression via Src-FAK-STAT3 Signaling in Lymphatic Endothelial Cells. [pubmed:27383632] |
| Oncology?Letters | RNAi-mediated?gene?silencing?of?vascular?endothelial?growth?factor?C?suppresses?growth?andinduces?apoptosis?in?mouse?breast?cancer?in?vitro?and in?vivo. [pubmed:27895746] |
| Angiogenesis | Characterization of isolated liver sinusoidal endothelial cells for liver bioengineering [Pubmed:29582235] |
| Oncology Reports | Effects of diphyllin as a novel V-ATPase inhibitor on TE-1 and ECA-109 cells [Pubmed:29328465] |
| LABORATORY INVESTIGATION | Dynamic signature of lymphangiogenesis during acute kidney injury and chronic kidney disease [Pubmed: 31019289] |
| Cutaneous and Ocular Toxicology | Isotretinoin does not alter VEGF-A and VEGF-C levels: Do retinoids behave differently in dose-dependent and/or in vivo/in vitro conditions? [Pubmed: 32722957] |
