結(jié)合珠蛋白(Hpt)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Haptoglobin (Hpt) ,etc. by FLIA (Flow Luminescence Immunoassay)
HP; Hp2-Alpha; Alpha-2-Macroglobulin; Zonulin
（注：?jiǎn)未位鞙y(cè)多因子不超過8個(gè)指標(biāo) ）
- 編號(hào)ULMA817Bo
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3.5h
- 檢測(cè)范圍0.98-1000ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.327 ng/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
- 價(jià)格 ¥ 1415 ¥ 1469 ¥ 1551 ¥ 1660 ¥ 1769 ¥ 1932 ¥ 2177 ¥ 2721 添加到價(jià)格計(jì)算器 計(jì)算器
- 欲了解實(shí)際交易價(jià)格和更多情況，請(qǐng)與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)結(jié)合珠蛋白(Hpt)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的結(jié)合珠蛋白(Hpt)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 80-95 | 83 |
| EDTA plasma(n=5) | 96-103 | 99 |
| heparin plasma(n=5) | 81-96 | 90 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的結(jié)合珠蛋白(Hpt)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中結(jié)合珠蛋白(Hpt)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-104% | 87-101% | 85-93% | 80-96% |
| EDTA plasma(n=5) | 92-101% | 89-96% | 95-103% | 80-97% |
| heparin plasma(n=5) | 86-104% | 81-96% | 98-105% | 96-103% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測(cè)溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測(cè)溶液B100μL，37°C振動(dòng)孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將結(jié)合珠蛋白(Hpt)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的結(jié)合珠蛋白(Hpt)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的結(jié)合珠蛋白(Hpt)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的結(jié)合珠蛋白(Hpt)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈(zèng)品
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA817Bo02 | 結(jié)合珠蛋白(Hpt)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA817Bo01 | 結(jié)合珠蛋白(Hpt)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA817Bo01 | 結(jié)合珠蛋白(Hpt)多克隆抗體 | WB; IHC |
| UPAA817Bo02 | 結(jié)合珠蛋白(Hpt)多克隆抗體 | IHC |
| UMAA817Bo21 | 結(jié)合珠蛋白(Hpt)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA817Bo | 結(jié)合珠蛋白(Hpt)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA817Bo | 結(jié)合珠蛋白(Hpt)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Vascular Surgery | Circulation levels of acute phase proteins in patients with Takayasu arteritis [PubMed: 20100644] |
| Genetics | Quantitative trait loci mapping of the mouse plasma proteome (pQTL). [NCBI: PMC3567747] |
| ostaglandins Leukot Essent Fatty Acids. | Docosahexaenoic and eicosapentaenoic acid supplementation does not exacerbate oxidative stress or intravascular haemolysis in homozygous sickle cell patients. [Pubmed: 24095588] |
| PLoS One | Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study [PubMed: 25955845] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
| Annals of Neurology? | High complement levels in astrocyte‐derived exosomes of Alzheimer disease [Pubmed:29406582] |
| European Journal of Medicinal Plants | Effects of Hippophae rhamnoides L. Leaf and Marc Extract with Reduced Tannin Concentration on the Health and Growth Parameters of Newborn Calves [] |
| Journal?of?Animal?Science | Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood?… [Pubmed: 30184125] |
| Journal of Clinical Medicine | Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients [Pubmed: 30486327] |
| International Journal of Biological Sciences | Generation of Pigs Resistant to Highly Pathogenic-Porcine Reproductive and Respiratory Syndrome Virus through Gene Editing of CD163 [] |
| Food and Chemical Toxicology | Prolyl endopeptidase-degraded low immunoreactive wheat flour attenuates immune responses in Caco-2 intestinal cells and gluten-sensitized BALB/c mice [Pubmed: 31082461] |
| Journal of Animal Science | Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers [Pubmed: 31410465] |
| International Journal of Biological Sciences | Deletion of CD163 Exon 7 Confers Resistance to Highly Pathogenic Porcine Reproductive and Respiratory Viruses on Pigs [] |
| Pediatric Obesity | Intestinal permeability and small intestine bacterial overgrowth in excess weight adolescents [Pubmed: 33089672] |
| JOURNAL OF ANIMAL SCIENCE | Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient??- [33474564] |
| large animal review | Influence of niacin application on inflammatory parameters, non-esterified fatty acids and functional status of liver in cows during early lactation [] |
| PLoS One | Could serum zonulin be an intestinal permeability marker in diabetes kidney disease? [34170951] |
| Probiotics Antimicrob Proteins | The Effect of Feed Supplementation with EM Bokashi? Multimicrobial Probiotic Preparation on Selected Parameters of Sow Colostrum and Milk as Indicators?… [34596883] |
