神經(jīng)生長因子(NGF)單克隆抗體
Monoclonal Antibody to Nerve Growth Factor (NGF)
NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
- 編號UMAA105Ra22
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C2
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA105Ra01-神經(jīng)生長因子(NGF)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Homo sapiens (Human，人)
- 應(yīng)用WB; IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 335 ¥ 781 ¥ 1116 ¥ 2790 ¥ 11160
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Rat Cerebrum Tissue; Primary Ab: 20μg/ml Mouse Anti-Rat NGF Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue
Primary Ab: 20μg/ml Mouse Anti-Rat NGF Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Hela cell lysate Primary Ab: 2μg/ml Mouse Anti-Rat NGF Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對NGF的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別NGF。
用法
Western blottin:g: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA105Ra01 | 神經(jīng)生長因子(NGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA105Ra01 | 神經(jīng)生長因子(NGF)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA105Ra61 | 神經(jīng)生長因子(NGF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA105Ra02 | 神經(jīng)生長因子(NGF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA105Ra01 | 神經(jīng)生長因子(NGF)多克隆抗體 | WB; IHC; ICC/IF |
| UMAA105Ra22 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB; IHC |
| UMAA105Ra21 | 神經(jīng)生長因子(NGF)單克隆抗體 | WB |
| USEA105Ra | 神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UWEA105Ra | 神經(jīng)生長因子(NGF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,寬范圍) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA105Ra | 神經(jīng)生長因子(NGF)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA105Ra | 神經(jīng)生長因子(NGF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA105Ra01 | 神經(jīng)生長因子(NGF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Yonsei Medical Journal | NGF and HB-EGF: Potential Biomarkers that Reflect the Effects of Fesoterodine in Patients with Overactive Bladder Syndrome [Pubmed:25510766] |
| Molecular and Cellular Endocrinology | High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96 [Pubmed:25614971] |
| Mol Cell Endocrinol | High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 [PubMed: 25614971] |
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| MOLECULAR NUTRITION & FOOD RESEARCH | Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and?… [Pubmed: 32918393] |
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| Pharmaceuticals (Basel) | Carissa macrocarpa Leaves Polar Fraction Ameliorates Doxorubicin-Induced Neurotoxicity in Rats via Downregulating the Oxidative Stress and Inflammatory Markers [34959705] |
| Cell Biochem Funct | CNTs‐CaP/chitosan‐coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway [34296452] |
| Carbohydrate Polymers | Gene delivery of chitosan-graft-polyethyleneimine vectors loaded on scaffolds for nerve regeneration [Pubmed:35550777] |
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